{"id":520330,"date":"2025-05-19T15:12:58","date_gmt":"2025-05-19T13:12:58","guid":{"rendered":"https:\/\/www.phytocontrol.com\/?p=520330"},"modified":"2026-03-13T14:38:19","modified_gmt":"2026-03-13T13:38:19","slug":"cyanotoxin-analysis-greater-sensitivity-and-a-comprehensive-overview-520330","status":"publish","type":"post","link":"https:\/\/stagingphytocontrol.shdesign.fr\/en\/news\/cyanotoxin-analysis-greater-sensitivity-and-a-comprehensive-overview-520330\/","title":{"rendered":"Cyanotoxin analysis: greater sensitivity and a comprehensive overview"},"content":{"rendered":"\n\n\n<p><b>The Phytocontrol laboratory offers cyanotoxin analysis using LC-MS\/MS, a benchmark technique for detecting microcystins in fresh water.<\/b><\/p>\n\n\n\n<p>Our R&amp;D department has just taken a new step forward with <b>two major developments to this method<\/b>, enabling us to respond even more precisely to regulatory requirements and practical needs.<\/p>\n\n\n\n<p>\u00a0<\/p>\n\n\n\n<p>\u00a0<\/p>\n\n\n\n<p>Following regulatory changes, we have<b> lowered the quantification limit to 0.1 \u00b5g\/L<\/b> for <b>extracellular microcystins (dissolved form)<\/b>. The evolution of our method reinforces our reliability, robustness, and reproducibility, and allows us to be fully compliant with current regulations, in particular the recommendations of the <b>DGS<\/b>, which set an <b>alert threshold of 0.3 \u00b5g\/L<\/b> for microcystins in bathing water.<\/p>\n\n\n\n<p>Initially limited to <b>extracellular<\/b> analysis, our method can now also analyze the <b>intracellular fraction<\/b>. This development provides a measurement of <b>total cyanotoxins<\/b>, giving a more complete picture of the contamination of a sample.<\/p>\n\n\n\n<p><b>For treated fresh water<\/b>, our COFRAC-accredited cyanotoxin analysis method provides results in the <b>extracellular fraction<\/b> only.<\/p>\n\n\n\n<p><b>However, for untreated fresh water<\/b>, the results can be expressed either as <b>extracellular (dissolved) fraction<\/b> or as <b>total cyanotoxins<\/b> (including extracellular and intracellular fractions).<\/p>\n\n\n\n<p>\u00a0<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">What is the regulatory context?<\/h3>\n\n\n\n<p>Cyanotoxin analysis is subject to a clear regulatory framework, particularly for bathing water, according to <b>instruction DGS\/EA4\/EA3\/2021\/76<\/b>:<\/p>\n\n\n\n<p>In the presence of <b>chlorophyll a &gt; 10 \u00b5g\/L<\/b>, <b>cyanobacteria identification<\/b> is required.<\/p>\n\n\n\n<p>If potentially toxinogenic genera are present, a <b>biovolume count<\/b> is performed (results are expressed in biovolumes):<\/p>\n\n\n\n<ul class=\"wp-block-list\"> <li><b>No alert<\/b> if the sum of cyanobacteria biovolumes \u2264 1 mm3\/L<\/li> <\/ul>\n\n\n\n<ul class=\"wp-block-list\"> <li><b>Level 1 alert<\/b> if the sum of cyanobacterial biovolumes &gt; 1 mm3\/L and toxin thresholds are not exceeded: search for toxins and maintain normal activity on the site. Inform the public.<\/li> <\/ul>\n\n\n\n<ul class=\"wp-block-list\"> <li><b>Level 2 alert<\/b> if the sum of cyanobacterial biovolumes &gt; 1 mm3\/L and one of the following toxin thresholds is exceeded:<\/li> <\/ul>\n\n\n\n<ul class=\"wp-block-list\"> <li>Microcystin &gt; 0.3 \u00b5g\/L<\/li> <\/ul>\n\n\n\n<ul class=\"wp-block-list\"> <li>Saxitoxin &gt; 30 \u00b5g\/L<\/li> <\/ul>\n\n\n\n<ul class=\"wp-block-list\"> <li>Cylindrospermopsin &gt; 42 \u00b5g\/L<\/li> <\/ul>\n\n\n\n<ul class=\"wp-block-list\"> <li>Anatoxin &gt; LD<\/li> <\/ul>\n\n\n\n<p>In the event of an alert: swimming is prohibited, the public is informed, water activities are restricted, and fish consumption is discouraged.<\/p>\n\n\n\n<p>Need technical, regulatory, or pricing information? Our customer service team is available Monday through Friday from 8 a.m. to 6:30 p.m.<\/p>\n\n\n\n<p>And to stay up to date with all the latest news from Phytocontrol, join us on our <a href=\"https:\/\/www.linkedin.com\/company\/laboratoire-phytocontrol\/\">LinkedIn<\/a> page!<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The Phytocontrol laboratory offers cyanotoxin analysis using LC-MS\/MS, a benchmark technique for detecting microcystins in&hellip;<\/p>\n","protected":false},"author":8,"featured_media":520322,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[180],"tags":[],"class_list":["post-520330","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-news"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.3 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Cyanotoxin analysis: greater sensitivity and a comprehensive overview - 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